![]() Hepatocyte growth factor/scatter factor induces a variety of tissue-specific morphogenic programs in epithelial cells. ![]() ![]() Brinkmann V, Foroutan H, Sachs M, Weidner KM, Birchmeier W.CD66 receptor specificity exhibited by neisserial Opa variants is controlled by protein determinants in CD66 N-domains. Bos MP, Kuroki M, Krop-Watorek A, Hogan D, Belland RJ.The organizing principle: Microenvironmental influences in the normal and malignant breast. Bissell MJ, Radisky DC, Rizki A, Weaver VM, Petersen OW.simulates in vivo growth patterns and allows discrimination of cell type. Culture of normal and malignant primary human mammary epithelial cells in a physiological manner. Carcinoembryonic antigens: Alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family. Barnett TR, Kretschmer A, Austen DA, Goebel SJ, Hart JT, Elting JJ, Kamarck ME. ![]() Human biliary glycoprotein gene: Characterization of a family of novel alternatively spliced RNAs and their expressed proteins. Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane. Barcellos-Hoff MH, Aggeler J, Ram TG, Bissell MJ.Thus, CEACAM1 is a critical molecule in mammary morphogenesis and may play a role in the absorption of the lipid vesicles of milk in the infant intestinal tract. Immuno-electron microscopy reveals numerous vesicles coated with CEACAM1 within the lumena, and as predicted by this finding, CEACAM1 is found in the lipid fraction of breast milk. CEACAM1 directly associates with and down-regulates the expression of β1-integrin. MCF7 cells that do not express CEACAM1 and fail to form lumena in Matrigel, revert to a lumen forming phenotype when transfected with the CEACAM1-4S but not the -4L isoform. The 'normal' breast epithelial line MCF10F forms acini with lumena in Matrigel with apical membrane expression of CEACAM1. Although most invasive ductal carcinomas express CEACAM1 (21/26), the staining pattern tends to be weak and cytoplasmic in tumours with minimal lumena formation (grades 2–3), while there is membrane staining in well-differentiated tumours (grade 1). ![]() Strong lumenal membrane staining is observed in 100% of normal glands (11/11), low in atypical hyperplasia (2/6), high in cribiform ductal carcinoma in situ (DCIS) (8/8), but low in other types of DCIS (2/15). CEA cell adhesion molecule-1 (CEACAM1) is a cell–cell adhesion molecule that, paradoxically, is expressed in an apical location in normal breast epithelium. ![]()
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